rabbit polyclonal anti brevican  (Developmental Studies Hybridoma Bank)

Journal: Biomedical Reports

Article Title: Prolonged use of temozolomide leads to increased anxiety and decreased content of aggrecan and chondroitin sulfate in brain tissues of aged rats

doi: 10.3892/br.2023.1695

Figure Lengend Snippet: Sequences of primers used in reverse transcription-quantitative PCR analysis.

Article Snippet: The following primary antibodies were used for immunostaining: mouse monoclonal anti-CS (1:100; cat. no. C8035; Sigma-Aldrich; Merck KGaA), rabbit polyclonal anti-aggrecan (1:100; cat. no. ab36861; Abcam), rabbit polyclonal anti-decorin (1:100; cat. no. ab175404; Abcam), and rabbit polyclonal anti-brevican (1:100; cat. no. ab111719; Abcam) for 1 h at room temperature; mouse monoclonal anti-GFAP (ready-to-use; cat. no. MS-1376-R7; Thermo Fisher Scientific) and mouse monoclonal anti-Olig2 (ready-to-use; cat. no. 211F1.1; Cell Marque) for 30 min at room temperature The staining patterns for CS, aggrecan, decorin and brevican were visualized with Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (cat. no. ab64264; Abcam).

Techniques: Sequencing

Journal: Biomedical Reports

Article Title: Prolonged use of temozolomide leads to increased anxiety and decreased content of aggrecan and chondroitin sulfate in brain tissues of aged rats

doi: 10.3892/br.2023.1695

Figure Lengend Snippet: Expression of HSPGs and СSPGs core proteins in rat brain tissues of the control, TMZ-treated, DXM-treated and TMZ + DXM-treated experimental animals. Reverse transcription-quantitative PCR, expression normalized to that of GAPDH (OriginPro 8.5). The bars represent the mean ± standard deviation from triplicate experiments (OriginPro 8.5). ANOVA + Fisher's Least Significant Difference test; * P<0.05. Control, non-treated rat brain tissue; TMZ, temozolomide; DXM, dexamethasone; HSPG, heparan sulfate proteoglycan; CSPG, chondroitin sulfate proteoglycan; Sdc1, syndecan-1; Sdc3, syndecan-3; Gpc1, glypican-1; Hspg2, heparan sulfate proteoglycan 2/perlecan; Dcn, decorin; Bgn, biglycan; Lum, lumican; Bcan, brevican; Ncan, neurocan; Acan, aggrecan; Vcan, versican; Cspg4/Ng2, chondroitin sulfate proteoglycan 4; Cspg5, chondroitin sulfate proteoglycan 5; Ptprz1, proteintyrosine phosphatase receptor type Z1/phosphacan.

Article Snippet: The following primary antibodies were used for immunostaining: mouse monoclonal anti-CS (1:100; cat. no. C8035; Sigma-Aldrich; Merck KGaA), rabbit polyclonal anti-aggrecan (1:100; cat. no. ab36861; Abcam), rabbit polyclonal anti-decorin (1:100; cat. no. ab175404; Abcam), and rabbit polyclonal anti-brevican (1:100; cat. no. ab111719; Abcam) for 1 h at room temperature; mouse monoclonal anti-GFAP (ready-to-use; cat. no. MS-1376-R7; Thermo Fisher Scientific) and mouse monoclonal anti-Olig2 (ready-to-use; cat. no. 211F1.1; Cell Marque) for 30 min at room temperature The staining patterns for CS, aggrecan, decorin and brevican were visualized with Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (cat. no. ab64264; Abcam).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Biomedical Reports

Article Title: Prolonged use of temozolomide leads to increased anxiety and decreased content of aggrecan and chondroitin sulfate in brain tissues of aged rats

doi: 10.3892/br.2023.1695

Figure Lengend Snippet: Immunohistochemical analysis of aggrecan, decorin and brevican content in the control and TMZ/DXM-treated experimental rats. Quantitative analysis of aggrecan, decorin and brevican content was performed with ZENblue software (magnification, x400; scale bar, 50 µm). The bars represent the mean ± standard deviation from triplicate experiments (OriginPro 8.5). ANOVA + Fisher's Least Significant Difference test; * P<0.05. Control, non-treated rat brain tissue; TMZ, temozolomide; DXM, dexamethasone; Acan, aggrecan; Dcn, decorin; Bcan, brevican; DAB, 3,3'-diaminobenzidine.

Article Snippet: The following primary antibodies were used for immunostaining: mouse monoclonal anti-CS (1:100; cat. no. C8035; Sigma-Aldrich; Merck KGaA), rabbit polyclonal anti-aggrecan (1:100; cat. no. ab36861; Abcam), rabbit polyclonal anti-decorin (1:100; cat. no. ab175404; Abcam), and rabbit polyclonal anti-brevican (1:100; cat. no. ab111719; Abcam) for 1 h at room temperature; mouse monoclonal anti-GFAP (ready-to-use; cat. no. MS-1376-R7; Thermo Fisher Scientific) and mouse monoclonal anti-Olig2 (ready-to-use; cat. no. 211F1.1; Cell Marque) for 30 min at room temperature The staining patterns for CS, aggrecan, decorin and brevican were visualized with Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (cat. no. ab64264; Abcam).

Techniques: Immunohistochemistry, Software, Standard Deviation

Journal: Molecular medicine reports

Article Title: Local intra‑articular injection of vascular endothelial growth factor accelerates articular cartilage degeneration in rat osteoarthritis model.

doi: 10.3892/mmr.2018.8652

Figure Lengend Snippet: Figure 4. Protein expression levels of aggrecan and type II collagen in rat condylar cartilage at 12 weeks after anterior cruciate ligament transection. (A) Representative western blot analyses of aggrecan and type II collagen protein expression; GAPDH was used as a loading control. (B and C) Densitometric analysis of protein expression of (B) aggrecan and (C) type II collagen. Data were normalized to the Control group and presented as the mean ± standard deviation; *P<0.05 vs. Control; #P<0.05 vs. NS. NS, normal saline; VEGF, vascular endothelial growth factor.

Article Snippet: Mouse anti-collagen II monoclonal antibody (sc-52658) and rabbit anti-aggrecan polyclonal antibody (sc-166951) were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Western Blot, Control, Standard Deviation, Saline